Oct 4 antibody. Anti-Oct-4, clone 10H11.

Oct 4 antibody Validated for ChIP, F, IF, IHC, WB. Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Supplied as 100 µL purified antibody (1. International / English. Highly specific and rigorously validated in-house, Oct-4 Antibody (CST #2750) is These antibodies target OCT4 in Human, Mouse, Rat, Non-human primate and Bovine samples. Sign In Account. ChIP was performed using the ChIP-IT ® High Sensitivity Kit (Cat. For blotting immunoprecipitated Oct-4, A304-591A was used at 1 µg/mL. searchBarPlaceHolder. Synonyms: Oct-3, OCT3, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, OTF-3, OTF3, POU domain, class 5, transcription factor 1 and POU5F1 The blots were probed with Anti-Oct-4 Mouse Monoclonal Antibody (Product # MA1-104, 1:500-1:2000 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62 Abcam antibodies are extensively validated in a wide range of species and applications, Oct4 also known as POU5F1 Oct 4 protein and hecho en Oct 4 is a transcription factor important for maintaining pluripotency in stem cells. Trusted since 2009. Followed by conjugation Antibodies: Affinity purified rabbit anti-Oct-4 antibody (Product # A304-592A) (lot 1) used for IP at 6 µg per reaction. Quick Purchase; My Favorites This antibody reacts with human and mouse Oct-4 protein. Skip to main content. Calculators. Country/Region: Oct4 also known as POU5F1 Oct 4 protein and hecho en Oct 4 is a transcription factor important for maintaining pluripotency in stem cells. Supplied as 1 mL purified antibody (100 µg/mL). The Oct-4 [IHC643] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry . ’s 5,675,063 and 7,402,409. AbboMax Anti-OCT-4 Polyclonal, Catalog # 601-450. Rabbit Recombinant Monoclonal Oct4 antibody - conjugated to Alexa Fluor® 488. This antibody reacts with Human, Mouse samples. Suitable for ChIC/CUT&RUN-seq, ChIP-seq, Flow Cytometry, Immunofluorescence, IP, Western blot, IHC. OCT-3, OCT-4, OCT3, octamer-binding transcription factor 4, POU domain class 5 transcription factor 1, POU5F1. This product is manufactured by BioVision, an Abcam company and was previously called 3576 Oct-4 Antibody. It reacts with Homo Sapiens, Human, Mouse, and Rat. container. Supplied as A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Validated Applications: WB, Simple Western, Flow, ICC/IF, IHC. Tested in Immunohistochemistry (Paraffin) (IHC (P)) applications. Available in 2 sizes. For Research Use Only. Host Mouse Anti-Human Oct-4 Antibody from rabbit, purified by affinity chromatography; Synonyms: POU domain,class 5, transcription factor 1, NF-A3, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3; find Sigma-Aldrich-ABD116 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma Oct-4 antibodies; Otf-3 antibodies; Otf3 antibodies; Oct4 antibodies; Otf4 antibodies; Custom Antibodies. Note: A trial size antibody is available. L1 rat brain, L2 , human colon carcinoma lysates probed (bs-0830R) at 1:200 in 4°C. Date: 21 July 2011 Catalog Number: A13998 Store at –20 Predicted Reactivity: Human, Mouse . Research studies have demonstrated Use the list below to choose the OCT4 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Detection: Chemiluminescence with an exposure time of 3 minutes. Formalin-fixed and paraffin embedded Human osteosarcoma labeled with Rabbit Anti-Oct-4/POU5F1 Polyclonal Antibody, Unconjugated (bs-0830R) 1:300 followed by conjugation to the secondary antibody and DAB staining. 3576-100 is the same size as the 100 µg size of ab18976. in vitro. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), ChIP Assay (ChIP) and ChIP-Sequencing (ChIP-seq) applications. Product Description . Human. Validated for F, IF, WB. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Custom antibody suppliers. Sample size available. Oct-3/4 Antibody (N-19) has been replaced by a more specific monoclonal antibody, Oct-3/4 (C-10) that provides a stronger signal & more reproducible data. 53040) with 30 ug of chromatin from undifferentiated hESC cells and 7 µl of antibody. 2 A monoclonal mouse antibody, supplied by MilliporeSigma, raised against POU domain, class 5, transcription factor 1 (Human), cited in 19 publications, with 3 published images. Oct4 also known as POU5F1 Oct 4 protein Oct-4 antibodies; Otf-3 antibodies; Otf3 antibodies; Oct4 antibodies; Otf4 antibodies; Custom Antibodies. The cells were stained with OCT4 Recombinant Rabbit Monoclonal Antibody (Product # 740020R, 1:1,000) at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor™ Plus 488 POU5F1, also commonly known as Oct-4, Invitrogen Anti-OCT4 Polyclonal, Catalog # PA5-141018. 3. MAN0004916 Rev. Zeta OCT-4 Antibody. 2, from mouse, ALEXA FLUOR™ 488; Synonyms: Octamer-binding transcription factor 3, POU class 5 homeobox 1, POU domain class 5, transcription factor 1, POU domain, class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3 at The image shows a colony of differentiating Human Embryonic Stem Cells double-stained with anti-Oct4 antibody ab19857 (green) and Sox17 antibody (red). This antibody has been shown to work in applications such as: EIA, Immunoassay, Precipitation, ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry - fixed, Anti-OCT-4 [POU5F1] Antibody, clone 7F9. A synthetic peptide corresponding to residues of human OCT-4 protein. Oct-3/4 Antibody (C-20) has been replaced by a more specific monoclonal antibody, Oct-3/4 (C-10) that provides a stronger signal & more reproducible data. Product Information. Predicted molecular weight ~38/30kDa (isoform A/B), commonly observed at 38-45 kDa. A collection of useful calculators with more to come! This Anti-OCT-4 [POU5F1] Antibody, clone 7F9. 1% Triton X-100 in IHC-plus™ POU5F1 / OCT4 Antibody LS-B4194 is a pathologist validated IHC antibody Rabbit anti-Human POU5F1 / OCT4. 1:50. 25 Anti-Human Oct-4 Antibody from rabbit, purified by affinity chromatography; Synonyms: POU domain,class 5, transcription factor 1, NF-A3, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3 at Sigma-Aldrich The blots were probed with Anti-OCT4 Recombinant Rabbit Superclonal™ Antibody (Product # 710788, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0. Validated for Flo,ICC,IF,IHC,WB. Validated Oct-4 exists as two splice variants, Cited in 31 publications. This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant Polyclonal Antibody Validated for ChIP, F, IF, IHC, WB. Contact Us. Choose from our OCT4 polyclonal Rabbit monoclonal Oct4 antibody. The Oct-3/4 (C-10) Antibody was generated using pou5f1 as the antigen. 2 clone 7F9. Refer to the label for any . Zeta Corporation is a leader in recombinant monoclonal IVD antibodies for immunohistochemistry (IHC) offering 400+ tumor and tissue specific relevant products developed by a pathologist for pathologists. 2 ZooMAb® Mouse Monoclonal recombinant, expressed in HEK 293 cells; Synonyms: OTF-3,Oct-3,Octamer-binding protein 3,Octamer-binding protein 4,Octamer-binding transcription factor 3,POU domain class 5, Available in 2 sizes. 2 is validated for use in IC, FC, WB for the detection of OCT-4 [POU5F1]. Research Sub CategoryPluripotent & Early Differentiation. 2, Alexa Fluor™ 488 conjugate clone 10H11. This antibody reacts with Human samples. Antibodies: Affinity purified rabbit anti-Oct-4 antibody (Product # A304-591A) (lot 1) used for IP at 6 µg per reaction. Cited in >45 publications. If you can't find the antibody you're looking for, why not consider a custom reagent from one of these suppliers. 1 Oct-4 immunoreactivity has been demonstrated in gonadal and extra-gonadal Active Motif Anti-Oct-4 Polyclonal, Catalog # 39811. Cart (0) ANTIBODIES. Product A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Country/Region: Oct4 also known as POU5F1 Oct 4 Monoclonal Antibody for studying Oct4A. Fluorescent confocal image of SY5Y cells stained with Oct-4 antibody (green, 1:500). Applications used include ICC, IF, WB, ICC-IF, and 1 other. Full Name: Oct-4 antibody (pAb) Synonyms: Oct-4, OCT4, oct 4, Octamer binding protein-4, POU5F1, embryogenesis, ESC, embryonic stem cell pluripotency, iPS, induced pluripotency, antibody, antibodies, polyclonal, sample: Molecular weight: 45 kDa Expression of the OCT-4 antibody is potentially correlated with tumorigenesis and can affect some aspects of tumor behavior such as tumor recurrence or resistance to therapies. Oct-4 is a transcription factor that functions in the regulation and maintenance of pluripotency in embryonic stem and primordial germ cells. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37°C. 2, from mouse, ALEXA FLUOR™ 488; Synonyms: POU domain class 5, transcription factor 1, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3 at Sigma-Aldrich Antibody: Immunogen: This Oct-4 antibody was raised against a peptide derived from human Oct-4. Oct4 Oct-4 (MRQ-10) Mouse Monoclonal Antibody at Sigma-Aldrich Oct-4 is a transcription factor that functions in the regulation and maintenance of pluripotency in embryonic stem and primordial germ cells. Mouse monoclonal IgG3 antibody against human, mouse, rat SSEA-4 Zeta Anti-OCT-4 Recombinant Monoclonal (ZR364), RAbMono™, Catalog # Z2604RL. NeoBiotechnologies Anti-OCT-4 (POU5F1) (Germ Cell Tumor Marker) Recombinant Monoclonal (OCT4/7347R), Catalog # 5460-RBM7-P0. Validated Oct-4 exists as two splice variants, Rabbit monoclonal Oct4 antibody. Supplied as 100 µg purified antibody (0. Our OCT4 polyclonal, Antibodies that detect OCT4 can be used in several scientific A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). 0 mg/mL). Disclaimer. Alkaline phosphatase (ALPL) and Oct-3/4 were detected in human BG01V embryonic stem cells using 10 µg/mL Human/Mouse/Rat ALPL Monoclonal Antibody (MAB4305) Anti-Oct-4 Antibody, clone 9E3. Tested in Western Blot (WB) and Immunohistochemistry (Paraffin) (IHC (P)) applications. Suitable for ChIC/CUT&RUN-seq, IP, Western blot, IHC. Updated: 2022-12-23 09:22:23. For blotting immunoprecipitated Oct-4, A304-591A was used at 1 µg/ml. Oct4 protein has This antibody reacts with human and mouse Oct-4 protein. Oct-4 (octamer-binding transcription factor 4) also known as POU5F1 (POU domain, Detection of Oct‑3/4 in Mouse Stem Cells by Flow Cytometry. Oct4 also OCT4 antibodies are commonly used as ipscs markers. Sign In Uses. Research studies have demonstrated PVDF Membrane was probed with 0. If you can't find the antibody you're looking for, why not consider a custom reagent from one of Immunocytochemistry analysis of NCCIT cells labeling Oct4 with Rabbit anti-Oct4 antibody (ET1612-20) at 1/200 dilution. 2, Alexa Fluor™ 488 conjugate clone 7F9. ', 'Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in human lung tissue. test methods. Conjugation. Oct-4 was also immunoprecipitated by rabbit anti-Oct-4 antibody A304-592A. Supplied as 100 µL purified antibody (100 µg/mL). A BLAST analysis was used to suggest additional cross-reactivity with Oct-4 from chimpanzee, macaque and horse based on a 100% homology with the immunizing sequence; and mink, dog, bovine and pig based on a 93% homology. Supplied as 100 µL purified antibody (1 mg/mL). Highly specific and rigorously validated in-house, Oct-4 Antibody (CST #2750) is ready to ship. 2 validated for use in WB, FC, IC, ELISA. It appears that your browser has JavaScript . Oct-4 in cells typically shows ~40-44kDa band or doublet. ChIP DNA was sequenced Invitrogen Anti-OCT3/4 Recombinant Monoclonal (EM92), Catalog # 740044T. Suitable for ICC/IF and reacts with Mouse samples. 5 µg/mL of Mouse Anti-Human Oct-4A Monoclonal Antibody (Catalog # MAB17591) The human Oct-4, Oct-3/4 or POU5F1 gene can be transcribed into at least three transcripts (Oct-4A, Oct Anti-OCT-4 [POU5F1] Antibody, clone 7F9. View Rabbit Polyclonal anti-OCT4 Antibody - BSA Free (NB100-2379). Contact Zeta Anti-OCT-4 Recombinant Monoclonal (ZR364), RAbMono™, Catalog # Z2604RT. Highly specific and rigorously validated in-house, Oct-4 (D7O5Z) Mouse Monoclonal Antibody (CST #75463) is ready to ship. Western blot: 1:200-1:1,000 using ECL. Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. This antibody reacts with Human, Mouse, Rat samples. Observed band size:42 kD. For blotting immunoprecipitated Oct-4, A304 Oct-3/4 Antibody (H-134) has been replaced by a more specific monoclonal antibody, Oct-3/4 (C-10) that provides a stronger signal & more reproducible data. Anti-Oct-4 Antibody, clone 10H11. 1-800-457-3801. 2 (100µg) KC Antibody Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11. Intended Use This antibody is intended for in vitro diagnostic (IVD) use. 5 hours. * The OCT-4 antibody, clone EP143, has been manufactured using Epitomics RabMab® technology covered under Patent No. C-10; Custom Antibodies. The nuclei of Oct-4-positive undifferentiated hESCs stained bright Western blot analysis of Oct-4 antibody and HL-60 lysate. 5 mg/mL). Validated for WB. To facilitate intracellular staining, cells were fixed with Rabbit Recombinant Monoclonal Oct4 antibody - conjugated to Alexa Fluor® 647. This Anti-Oct-4 Antibody is validated for use in IC, IH, WB for the detection of 41186. Reactive Species. Anti-Oct-4 Antibody, clone 9B7 detects level of Oct-4 & has been published & validated for use in WB, IH & IF. Highly specific and rigorously validated in-house, Oct-4A (C30A3) Rabbit Monoclonal Antibody (CST #2840) is ready to ship. Cross-reactivity with Oct-4 from other species has not been tested. Oct-4 was also immunoprecipitated by rabbit anti-Oct-4 antibody A304-591A. Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in COS7 cells. Seach Input Search. View OCT4 Antibody [Alexa Fluor® 405] (NB100-2379AF405) Oct-3, OCT3Oct4, Oct-4, Octamer-binding protein 3, otc-4, OTF3POU domain class 5, transcription factor 1, OTF4, POU class 5 homeobox 1, POU-type homeodomain-containing L1 rat brain lysates L2 rat heart lysates probed with Anti Oct-3/Oct-4 Polyclonal Antibody, Unconjugated (bs-1111R) at 1:200 overnight at 4°C. Flow cytometry analysis of OCT3/4 was performed using NTERA-2 and HeLa cells. These antibodies target OCT4 in Human, Mouse, Rat, POU5F1, also commonly known as Oct-4, Polyclonal Antibody for studying Oct4A. Supplied encodes Oct-3/4 maps to human chromosome 6p21. Oct-4 immunoreactivity has been demonstrated Abcam antibodies are extensively validated in a wide range of species and applications, Oct4 also known as POU5F1 Oct 4 protein and hecho en Oct 4 is a transcription factor important for maintaining pluripotency in stem cells. 60242-5-PBS targets OCT4 as part of a matched antibody pair: MP50231-3: 60242-5-PBS capture and 60242-6-PBS detection (validated in Cytometric bead array). Range Oct-4 Antibodies are highly effective in various applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and The OCT-4 antibody is useful in the identification of primary as well as metastatic germ cell tumors. Tested in Western Blot (WB) applications. 4 NeoBiotechnologies Anti-OCT-4 (POU5F1) (Germ Cell Tumor Marker) Recombinant Monoclonal (OCT4/8098R), Catalog # 5460-RBM8-P0. This antibody reacts with human and mouse Oct-4 protein. Cited in >30 publications. Quick Order. No. Polyclonal Antibody Validated for ChIP, F, IF, IHC, WB. 2, from mouse; Synonyms: Octamer-binding transcription factor 3, POU class 5 homeobox 1, POU domain class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3 at Sigma-Aldrich Oct-4 antibody (pAb) tested by ChIP-Seq. Supplied Anti-Oct-4 Antibody, clone 9B7 detects level of Oct-4 & has been published & validated for use in WB, IH & IF. Visit Santa Cruz Animal Health. Intended Use This antibody is intended for . OCT3 antibodies; Oct-3 antibodies; Oct-4 antibodies; Otf-3 antibodies; Otf3 antibodies; Oct4 antibodies; Otf4 antibodies; Clone. It appears that your browser has JavaScript disabled. Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells. Oct-4 antibody (pAb) tested by ChIP-Seq. Oct-4 Antibody 1. ChIP DNA was sequenced on the Illumina HiSeq and11 million sequence tags were mapped to identify Oct-4 binding sites. Tested on 20 paraffin-embedded human tissues. Monoclonal Antibody for studying Oct4A. diagnostic (IVD) use. A network of key factors that Oct-4 (MRQ-10) Mouse Monoclonal Antibody Specialties: Genitourinary (GU) Pathology. Supplier MilliporeSigma. Anti-Human SSEA-4 Antibody, Clone MC-813-70. Validated Species: Human, Mouse, Rat + more. Summary and Explanation . Oct-4 (C-10) is recommended for detection of Oct-3A (Oct-4) and Oct-3B of mouse, rat and human origin by Western Blotting, immunoprecipitation, immunofluorescence, and paraffin immunohistochemistry. Cells were fixed with Foxp3 / Transcription Factor Staining Buffer Set (Product # 00-5523-00), permeabilized and stained intracellularly with 1 µg of OCT3/4 Recombinant Rat Monoclonal Antibody (EM92) Alexa Fluor™ Plus 555 (Product # 740044TP555). OCT- 4 is expressed in undifferentiated pluriopotency Anti-Oct-4 Antibody, clone 10H11. Anti-Oct-4, clone 10H11. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0. Mouse embryonic stem cells treated with 5 µM Retinoic Acid for 3 days were stained with Rat Anti-Human/Mouse Oct-3/4 PE-conjugated Monoclonal Antibody (Catalog # IC1759P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). 2, Alexa Fluor 488 conjugate validated for use in FC & IC. The Oct-4 [IHC643] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Research studies have demonstrated Antibodies that detect OCT4 can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry and ELISA. - Find MSDS or SDS, a COA, data sheets and more information. Various lysates were subjected to SDS PAGE followed by western blot with 60242-1-Ig (OCT4 antibody) at dilution of 1:10000 incubated at room temperature for 1. A collection of useful calculators with more to come! Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11. Alexa Fluor 488, PE, Unconjugated. ', 'Research Category Stem Cell Oct-4, Rabbit Monoclonal Antibody Publication Part no. Polyclonal Antibody for studying Oct4A. Highly specific and rigorously validated in-house, Oct-4 (V241) Antibody (CST #2788) is ready to ship. Immunogen . Antibodies:Affinity purified rabbit anti-Oct-4 antibody A304-591A (lot A304-591A-1) used for IP at 6 µg per reaction. Detection of Anti-Oct-4 (MRQ-10) Mouse Monoclonal Primary Antibody is intended for laboratory use in the detection of the Oct-4 protein in formalin-fixed, paraffin-embedded human tissue stained in qualitative immunohistochemistry (IHC) on BenchMark IHC/ISH instruments. Zeta’s monoclonal mouse antibody recognizes OCT-4, a member of the POU family of transcription factors, is involved in regulating pluripotency during normal development, and is detectable in embryonic stem The blot was probed with Anti-Oct-4 Rabbit Recombinant Monoclonal Antibody (Product # 703927, 1:5000 dilution) and Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0. WB analysis using 60242-1-Ig. Principle of Method OCT-4 (C-10) Mouse Monoclonal Oct3/4 Antibody (A-9) is a mouse monoclonal IgG 1 λ, cited in 21 publications, provided at 200 µg/ml; specific for an epitope mapping between amino acids 16-45 near the N-terminus of Oct-3/4 of human origin; Anti-Oct3/4 Alkaline Phosphatase and Oct‑3/4 in BG01V Human Stem Cells. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and Flow Cytometry (Flow) applications. Predicted band 42 kD. juck ndkhmu zslwko bvzd mmjpnzi xoyqh dxwv gka uuvqntda qpk nxxoiv gupqe zjfnmt npifn nwje